18 مرداد 1399

محسن صمیمی

مرتبه علمی:
نشانی:
تحصیلات: دکترای تخصصی
تلفن:
دانشکده: دانشکده انرژی

مشخصات پژوهش

عنوان
Characteristics of pDNA-loaded chitosan/alginate-dextran sulfate nanoparticles with high transfection efficiency
نوع پژوهش مقاله چاپ شده
کلیدواژه‌ها
Biopolymeric nanoparticles, dextran sulfate, transfection efficiency, cytotoxicity, carrier
پژوهشگران محسن صمیمی (نفر اول)، شامیل والیدف (نفر دوم)

چکیده

In this research pDNA-loaded chitosan/alginate-dextran sulfate nanoparticles with encapsulation efficiency from 83.07 to 94.71% were prepared using modified ionotropic gelation method. Encapsulated pDNA was tightly bound to polymers and protected from hydrolysis by a BamHI nuclease. All prepared nanoparticles were non-toxic for cells of HEK298 line. Moreover, chitosan/alginate-dextran sulfate nanoparticles increased proliferation of the cells in contrast to LipofectaminTM, used as a positive control for transfection. The particles prepared were effective in transfection of pEGFP-N2 plasmid into the human cells. High homogeneity of nanoparticles, reliable protection of the encapsulated DNA, and considerably high transfection efficiency along with absence of toxicity make DNA-loaded chitosan/alginate-dextran particles a suitable gene carriers in vitro and promising candidates for design of delivery systems for gene therapy.