Synthesis of new ionic liquids and the determination of their properties and applications are expanding in industrial fields. Despite significant advances, the knowledge of the physical, chemical and biological properties of many ionic liquids is still less than the current information on organic solvents. It is important to determine the properties of the ionic liquids and investigate the changes in these properties to synthesize the ionic liquids with required applications. In this study, a novel amine ionic liquid “Diethanolamine Lactic” [DEA][Lac] was synthesized. The replacement of halogenated ion fluid was used as a modified methodology for the preparation of diethanolamine based on lactic acid. The ionic liquid was characterized using the Fourier transform infrared (FTIR) spectra and the nuclear magnetic resonance (NMR) spectroscopy. The changes in bands' wavelengths and the peaks of the participating elements and process materials have been confirmed before and after synthesis based on the results of FTIR analysis and demonstrate the successful synthesis of diethanolamine lactic. The NMR analysis also clearly confirms the synthesis of diethanolamine lactic acid. Analysis results were shown in the successful synthesis of the [diethanolamine] [lactic].