In this experimental research, the laminar forced convection of water–magnetite nanofluid (NF) in a horizontal twisted tube (TT) is examined under a rotating magnetic field (MF). The findings are compared with those of the plain tube (PT). The influence of nanoadditive concentration ( ), Reynolds number (Re), twist pitch (P) and MF arrangement on the heat transfer, friction factor and overall thermohydraulic features of NF is assessed. The MF consists of two magnets that rotate around the tube. For each of the magnets, three modes of clockwise rotation, counterclockwise rotation and without rotation are considered. The findings showed that the combined use of TT and rotating MF entails an increase in the overall thermohydraulic features of water–magnetite NF. In addition, it was found that the overall thermohydraulic features of NF augment with boosting , while they decline with boosting P and Re. Moreover, it was revealed that the best thermohydraulic features of the water–magnetite NF belonged to the case of = 2%, Re = 500 and P = 10 mm in the presence of a rotating magnetic field resulting from the clockwise rotation of the first magnet and the counterclockwise rotation of the second magnet.