July 25, 2024
Mohsen Samimi

Mohsen Samimi

Academic rank: Associate professor
Address: Imam Khomeini Highway, Kermanshah, Iran
Education: Ph.D in Chemical Engineering - Biotechnology
Phone: 08338305001
Faculty: Faculty of Engineering

Research

Title
Characteristics of pDNA-loaded chitosan/alginate-dextran sulfate nanoparticles with high transfection efficiency
Type Article
Keywords
Biopolymeric nanoparticles, dextran sulfate, transfection efficiency, cytotoxicity, carrier
Researchers Mohsen Samimi، Shamil Validov

Abstract

In this research pDNA-loaded chitosan/alginate-dextran sulfate nanoparticles with encapsulation efficiency from 83.07 to 94.71% were prepared using modified ionotropic gelation method. Encapsulated pDNA was tightly bound to polymers and protected from hydrolysis by a BamHI nuclease. All prepared nanoparticles were non-toxic for cells of HEK298 line. Moreover, chitosan/alginate-dextran sulfate nanoparticles increased proliferation of the cells in contrast to LipofectaminTM, used as a positive control for transfection. The particles prepared were effective in transfection of pEGFP-N2 plasmid into the human cells. High homogeneity of nanoparticles, reliable protection of the encapsulated DNA, and considerably high transfection efficiency along with absence of toxicity make DNA-loaded chitosan/alginate-dextran particles a suitable gene carriers in vitro and promising candidates for design of delivery systems for gene therapy.